Investigation on Microbiologically Influenced Corrosion Behavior of CrCoNi Medium-Entropy Alloy byPseudomonas Aeruginosa

doi:10.11900/0412.1961.2019.00030

Acta Metall Sin

2019, Vol. 55

Issue (11): 1457-1468 DOI: 10.11900/0412.1961.2019.00030

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Investigation on Microbiologically Influenced Corrosion Behavior of CrCoNi Medium-Entropy Alloy byPseudomonas Aeruginosa

FENG Hao¹,LI Huabing¹(

),LU Pengchong¹,YANG Chuntian²,JIANG Zhouhua¹,WU Xiaolei³

1. School of Metallurgy, Northeastern University, Shenyang 110819, China
2. Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang 110819, China
3. State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China

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FENG Hao,LI Huabing,LU Pengchong,YANG Chuntian,JIANG Zhouhua,WU Xiaolei. Investigation on Microbiologically Influenced Corrosion Behavior of CrCoNi Medium-Entropy Alloy byPseudomonas Aeruginosa. Acta Metall Sin, 2019, 55(11): 1457-1468.

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Abstract

The CrCoNi medium-entropy alloy (MEA) has excellent strength and toughness, and can be used as the basis for the development of promising engineering alloys in the future. However, microbiologically influenced corrosion (MIC) of CrCoNi MEA has rarely been reported. Especially, pseudomonas aeruginosa (P. aeruginosa) is the typical bacteria associated with MIC, which is widely distributed in the ocean and soil. It can form biofilm on the surface of steel and accelerate the corrosion of carbon steels and stainless steels (SSs). In this study, the electrochemical experiments such as open current potential (OCP), linear polarization resistance (LPR), electrochemical frequency modulation (EFM), electrochemical impedance spectroscopy (EIS) and cyclic polarization (CP) were used to investigate the MIC behavior of CrCoNi MEA caused by P. aeruginosa, in comparison with 316L SS. Surface analysis techniques such as FESEM and CLSM were used to observe the P. aeruginosa biofilm and pitting morphology on the coupon surface. The results show that P. aeruginosa could form an uneven biofilm on the surface of CrCoNi MEA coupons. The P. aeruginosa accelerated the corrosion rate of CrCoNi MEA, which was demonstrated by a negative shift of open circuit potential, a decrease of polarization resistance and charge transfer resistance, and an increase of corrosion current density in P. aeruginosa medium. The P. aeruginosa biofilm could destroy the passive film of the CrCoNi MEA coupons, which led to the maximum pit depth of the coupons exposed in P. aeruginosa medium (4.8 μm) for 14 d much deeper than that in sterile medium (2.3 μm). Compared with 316L SS, CrCoNi had higher open circuit potential, lower corrosion current density and corrosion rate, and higher repairability of passive film. Meanwhile, the maximum pit depth on the CrCoNi MEA coupons in P. aeruginosa medium was shallower than that of 316L SS (5.8 μm).

Key words: CrCoNi medium-entropy alloy (MEA) pseudomonas aeruginosa microbiologically influenced corrosion (MIC) biofilm pitting corrosion

Received: 29 January 2019

ZTFLH:

TG178

Fund: National Natural Science Foundation of China(51434004);National Natural Science Foundation of China(51774074);National Natural Science Foundation of China(U1435205);National Natural Science Foundation of China(51434004、51774074和U1435205);Fundamental Research Funds for the Central Universities No(N172512033);and Transformation Project of Major Scientific and Technological Achievements in Shenyang City(Z17-5-003)

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	Hao FENG
	Huabing LI
	Pengchong LU
	Chuntian YANG
	Zhouhua JIANG
	Xiaolei WU

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https://www.ams.org.cn/EN/10.11900/0412.1961.2019.00030 OR https://www.ams.org.cn/EN/Y2019/V55/I11/1457

Table 1 Chemical compositions of CrCoNi medium-entropy alloy (MEA) and 316L stainless steel (SS) (mass fraction / %)

Fig.1 E_OCP (a), 1/R_p (b) and corrosion rate (c) vs exposure time for CrCoNi MEA and 316L SS in sterile medium and pseudomonas aeruginosa (P. aeruginosa) medium (E_OCP—open circuit potential, R_p—linear polarization resistance)

Fig.2 pH value change curves of the sterile medium and P. aeruginosa medium for 14 d

Fig.3 Nyquist (a, c, e) and Bode (b, d, f) plots of CrCoNi MEA in sterile medium (a, b) and P. aeruginosa medium (c, d), and 316L SS in P. aeruginosa medium (e, f) for different time (Z'—real part of impedance, Z"—imaginative part of impedance, f—frequency, Z—modulus impedance)

Fig.4 Electrochemical impedance spectroscopy (EIS) equivalent circuit diagram (R_s—resistance of solution, Q_f—capacitance of oxide film layer, R_f—resistance of oxide film layer, Q_dl—capacitance of electrical double-layer, R_ct—charge transfer resistance of electrical double-layer)

Table 2 Fitting parameters for EIS of CrCoNi MEA and 316L SS in sterile medium and P. aeruginosa medium

Fig.5 Cyclic poarization curves of CrCoNi MEA and 316L SS in sterile medium and P. aeruginosa medium for 7 d (a) and 14 d (b) (E_CP—potential, i—current density)

Table 3 Parameters obtained from cyclic polarization curves for CrCoNi MEA and 316L SS in sterile medium and P. aeruginosa medium for 7 d

Fig.6 Macroscopic morphologies after cyclic polarization of CrCoNi MEA (a) and 316L SS (b) in P. aeruginosa medium for 14 d

Fig.7 FESEM (a, b) and CLSM (c, d) images of biofilm of CrCoNi MEA (a, c) and 316L SS (b, d) in P. aeruginosa medium for 7 dColor online

Fig.8 FESEM (a, b) and CLSM (c, d) images of biofilm of CrCoNi MEA (a, c) and 316L SS (b, d) in P. aeruginosa medium for 14 dColor online

Fig.9 Images of the maximum pit depth measured by CLSM on coupon surface of CrCoNi MEA in sterile medium (a), CrCoNi MEA in P. aeruginosa medium (b), and 316L SS in P. aeruginosa medium (c) for 14 dColor online

Table 5 Pit depths of CrCoNi MEA and 316L SS in sterile medium and P. aeruginosa medium for 14 d (μm)

Fig.10 Cumulative probability plots for the pit depth of CrCoNi MEA and 316L SS in sterile medium and P. aeruginosa medium for 14 d

Fig.11 Gumbel probability plots for the pit depth of CrCoNi MEA and 316L SS in sterile medium and P. aeruginosa medium for 14 d

Table 4 Parameters obtained from cyclic polarization curves for CrCoNi MEA and 316L SS in sterile medium and P. aeruginosa medium for 14 d

Table 6 Gumbel distribution parameters of CrCoNi MEA and 316L SS in sterile medium and P. aeruginosa medium for 14 d

Fig.12 Probabilities of various pit depths of CrCoNi MEA and 316L SS in sterile medium and P. aeruginosa medium for 14 d

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