Nickel base single crystal superalloys are widely used to fabricate turbine blade materials, since they have high temperature capability, excellent mechanical and environmental properties. Re is known as a very efficient solid-solution element that increases high temperature strength, so novel single crystal superalloys contain a high level of Re content. Whereas enhancement of Re in the dendrite arms facilitates inhomogeneous distribution of other alloy elements, it become difficult and important to determine the heat treatment in Re-containing single crystal superalloys. Effects of heat treatments on microstructure and properties in a new Re-containing single crystal superalloy have been investigated in this work. The solidus and liquidus temperature measured by differential thermal analysis (DTA) is 1339 and 1371 ℃, respectively. The incipient melting temperature determined by metallographic testing method is in the range between 1305 and 1310 ℃. The results show that Ti resides preferentially followed by B and S in the incipient melting regions. γ' precipitates all display cubic after 4 h aged at 1080, 1100 and 1120 ℃ followed by air cooling (A.C.). The optimum heat treatment is 1290 ℃, 2 h+1320 ℃, 4 h, A.C.+1100 ℃, 4 h, A.C.+900 ℃, 24 h, A.C.. Microsegregation of alloy elements reduces significantly after this heat treatment. The rupture property performs well and the rupture life is up to 78.2 h under the condition of 1070 ℃ and 140 MPa.