Influences of Protein Adsorption on the in vitro Corrosion of Biomedical Metals

doi:10.11900/0412.1961.2020.00198

Acta Metall Sin

2021, Vol. 57

Issue (1): 1-15 DOI: 10.11900/0412.1961.2020.00198

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Influences of Protein Adsorption on the in vitro Corrosion of Biomedical Metals

WANG Luning¹^,²(

), LIU Lijun¹, YAN Yu¹^,³, YANG Kun¹, LU Lili¹

^1.Beijing Advanced Innovation Center for Materials Genome Engineering, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
^2.State Key Laboratory for Advanced Metals and Materials, University of Science and Technology Beijing, Beijing 100083, China
^3.Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China

Cite this article:

WANG Luning, LIU Lijun, YAN Yu, YANG Kun, LU Lili. Influences of Protein Adsorption on the in vitro Corrosion of Biomedical Metals. Acta Metall Sin, 2021, 57(1): 1-15.

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Abstract

Protein could adsorb on the surfaces when biomedical metals contact with body fluids and then affect the corrosion behavior of metals. In vitro results demonstrate that protein adsorption retards metal dissolution, while the detachment of metal-protein complex from the surface accelerates the corrosion or its deposition could impede the metal corrosion. Protein adsorption and its influences on the metal corrosion are related to many factors, such as the type and content of proteins as well as the pro-perty of metals. Therefore, consensus has not been made on the influences of protein on metal corrosion. However, as one of most important components in the body fluids, it should be taken into consideration for the effects of protein on the corrosion behavior of metals in vitro. So that we can find the discrepancy between in vivo and in vitro tests and find the suitable simulated environment in vitro. This will help predict reasonably the corrosion behavior of biomedical metals in the human body.

Key words: protein biomedical metal corrosion adsorption in vitro test

Received: 03 June 2020

ZTFLH:

TG146.13

Fund: National Key Research and Development Program of China(2016YFC251100);National Natural Science Foundation of China(51503014)

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https://www.ams.org.cn/EN/10.11900/0412.1961.2020.00198 OR https://www.ams.org.cn/EN/Y2021/V57/I1/1

Table 1 The in vitro and in vivo corrosion rates of Mg and Mg alloys^[3-7]

Fig.1 Comparisons of in vivo and in vitro corrosion rates of magnesium alloys (The columns without error bars are obtained from less than three sets of data)

Fluid	Na⁺	K⁺	Ca²⁺	Mg²⁺	Cl^-	HCO $3 -$	HPO $4 2 -$	H₂PO $4 -$	SO $4 2 -$
	mmol·L^-1	mmol·L^-1	mmol·L^-1	mmol·L^-1	mmol·L^-1	mmol·L^-1	mmol·L^-1	mmol·L^-1	mmol·L^-1
Blood plasma^[10,13]	134-143	3.5-4.7	2.1-2.7	1.5	100-108	25-30	1.0	-	0.5
Synovia fluids^[10]	133-139	3.5-4.5	1.2-2.4		87-138	-	-	-
SBF^[15]	142.0	5.0	2.5	1.5	148.0	4.2	1.0	-	0.5
PBS^[3]	154.1	4.1	-	-	140.6	-	8.1	1.5	-
HBSS^[3,12]	142.8	5.8	2.5	0.8	143.3	4.2	0.3	0.4	0.8
DMEM^[3,12]	155.3	5.3	1.8	0.8	115.7	44.1	0.9	-	0.8
Ringer??s^[14]	147	4.1	4.3	-	160	-	-	-	-
SBP^[11]	142.0	5.0	2.5	1.5	103.0	27.0	1.0	-	0.5
Fluid	Amino acids	Glucose	Uric acid	Vitamins	Phenol red	Albumin	IgG	Fibrinogen
	g·L^-1	g·L^-1	g·L^-1			g·L^-1	g·L^-1	g·L^-1
Blood plasma^[10,13]	20-51	650-966	30.5-70.7	-	-	37.6-54.9	6.4-13.5	2-4
Synovia fluids^[10]	-	-	39	-		6-10	1.47-4.62	-
SBF^[15]	-	-		-	-
PBS^[3]	-	-		-	-
HBSS^[3,12]	-	5.6		-	-
DMEM^[3,12]	10.6	25.0		0.15	0.04
Ringer??s^[14]	-	-		-	-
SBP^[11]	-	-		-	-

Table 2 Chemical components of blood plasma, synovia fluids, several commonly used simulated body fluids and simulated blood plasma^[3,10-15]

Table 3 Analyses of metals in saline and protein solutions after 16 h exposure^[29]

Fig.2 Schematic of BSA (PDB bank: 4f5s) structure (BSA—bovine serum albumin)

Fig.3 Optimized complexes of BSA on polystyrene (PS) surface (a) and BSA on the GeO₂ surface (b)^[39]

Table 4 The structure, size, molecular weight, and isoelectric point of different kinds of proteins^[42]

Fig.4 Protein adsorption on the materials in SBF

Table 5 Binding constants and saturation values for BSA and Fg^[44]

Fig.5 Schematic model of the BSA layer formed on stainless-steel surface in artificial seawater, showing a first organic layer strongly attached to the oxide surface, and a second layer of proteins, the cohesion of which is ensured by magnesium ions^[46]

Fig.6 AFM topographic images (a, c) and SKPFM images (b, d) of the CoCrMo alloy surface (a, b), and BSA on the CoCrMo alloy surface (c, d), the curves under each figure show the ups and downs of morphology and the potential of the green line on the corresponding image; and schematic of the adsorption of BSA on the CoCrMo surface which induces the gathering of free electros under SKPFM (e)^{(AFM—atomic force microscope, SKPFM—surface Kelvin potential force microscopy)^[36]}

Fig.7 Potentiodynamic polarization curves of 316L^[66] (a), CoCrMo^[66] (b), Ti-6Al-4V^[66] (c), and pure Zr^[28] (d) in PBS solutions with various BSA concentrations (0-4 g/L) (E—potential, I—current density)

Fig.8 Schematic illustrations of corrosion behaviors of M1A alloy in SBF and BSA-containing SBF (A-SBF)^[15]

Table 6 The effects of BSA on the in vitro corrosion rate of biomedical metals in different solutions

Fig.9 Theoretical model of charged double layer showing the transfer of charge at the metal/oxide/protein interfaces before (a) and during (b) the corrosion process^[55]

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