生物可降解纯<strong>Mg</strong>表面<strong>BA + Alg@Ca</strong>复合涂层的制备及其体外生物相容性

doi:10.11900/0412.1961.2024.00181

金属学报

2026, Vol. 62

Issue (4): 669-684 DOI: 10.11900/0412.1961.2024.00181

研究论文

本期目录 | 过刊浏览

生物可降解纯Mg表面BA + Alg@Ca复合涂层的制备及其体外生物相容性

梁涛¹^,², 杜云波¹, 陈协辉¹, 潘浩波²^,³(

)

^1.深圳市龙华区中心医院深圳 518110
^2.中国科学院深圳先进技术研究院深圳市海洋生物医用材料重点实验室深圳 518055
^3.深圳市中科海世御生物科技有限公司深圳 518044

Fabrication and in vitro Biocompatibility Evaluation of BA + Alg@Ca Composite Coatings on Biodegradable Pure Mg

LIANG Tao¹^,², DU Yunbo¹, CHEN Xiehui¹, PAN Haobo²^,³(

)

^1.Shenzhen Longhua Central Hospital, Shenzhen 518110, China
^2.Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
^3.Shenzhen Healthemes Biotechnology Co. Ltd. , Shenzhen 518044, China

引用本文:

梁涛, 杜云波, 陈协辉, 潘浩波. 生物可降解纯Mg表面BA + Alg@Ca复合涂层的制备及其体外生物相容性[J]. 金属学报, 2026, 62(4): 669-684.
Tao LIANG, Yunbo DU, Xiehui CHEN, Haobo PAN. Fabrication and in vitro Biocompatibility Evaluation of BA + Alg@Ca Composite Coatings on Biodegradable Pure Mg[J]. Acta Metall Sin, 2026, 62(4): 669-684.

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摘要:

感染性骨缺损一直是临床骨科治疗中的重点性疾病，设计兼具抗菌和成骨性能的骨组织工程生物材料对感染性骨缺损修复具有重要意义。本工作通过水热处理和浸涂法两步处理在纯Mg表面构建了BA + Alg@Ca复合涂层(其中，BA表示H₃BO₃水热溶液处理，Alg@Ca表示含CaG的Alg)，系统研究了复合涂层的形成机制及体外生物相容性。结果表明，BA + Alg@Ca复合涂层由3层结构组成，内层为Mg(OH)₂，中间过渡层为MgB₂O(OH)₆，外层为Alg@Ca涂层。浸泡实验和电化学实验结果表明，BA + Alg@Ca复合涂层具有良好的耐腐蚀性能。体外抗菌实验结果表明，由于[B(OH)₄]^-和海藻酸盐的协同作用，BA + Alg@Ca复合涂层抑制了革兰氏阳性菌和革兰氏阴性菌的生长。BA + Alg@Ca复合涂层的细胞活性和增殖状态正常，表现出良好的细胞相容性。此外，BA + Alg@Ca复合涂层能诱导巨噬细胞向M2表型抑炎方向极化，发挥抑炎作用。BA + Alg@Ca复合涂层改性可降解纯Mg材料有望用于临床感染性骨缺损修复。

关键词 ：纯Mg, H₃BO₃, 海藻酸钠, 抗菌性能, 生物相容性

Abstract：

Infected bone defect is one of the most common and serious diseases in orthopedic surgery. Hence, it is important to design bone tissue engineering biomaterials with both antibacterial and osteogenic properties for the repair of infected bone defects. Accordingly, in this study, BA + Alg@Ca composite coatings (BA represents H₃BO₃ hydrothermal treatment, Alg@Ca represents CaG-incorporated alginate) composed of an inner layer (Mg(OH)₂), a middle layer (MgB₂O(OH)₆), and an outer layer (Alg@Ca) were constructed on pure Mg by hydrothermal treatment and dip coating, and the formation mechanism and in vitro biocompatibility were systematically investigated. BA + Alg@Ca coatings dramatically improved the degradation resistance of pure Mg, revealing a lower pH value and released Mg²⁺ concentration in the immersion test as well as nobler open circuit potential, larger impedance modulus, and lower corrosion current density in the electrochemical evaluation. In vitro antibacterial examination showed that BA + Alg@Ca coatings effectively inhibited the growth of gram-positive and gram-negative bacteria due to the synergetic effects of [B(OH)₄]^- and alginate. BA + Alg@Ca coatings also demonstrated good cytocompatibility with normal cell viability and proliferation. Moreover, these coatings could induce macrophage polarization to the M2 phenotype (anti-inflammatory), suggesting that they resulted in favorably selective cell adhesion and antibacterial performance. Overall, the BA + Alg@Ca coating-modified pure Mg can potentially be used for the repair of infected bone defects in the clinic.

Key words： pure Mg H₃BO₃ sodium alginate antibacterial property biocompatibility

收稿日期: 2024-05-28

ZTFLH:

TG146.2

基金资助:国家自然科学基金项目(32161160327);广东省基础与应用基础研究基金项目(2022A1515110901);深圳市医学研究专项项目(B2302031)

通讯作者: 潘浩波，hb.pan@siat.ac.cn，主要从事生物活性玻璃的制备与生物学应用研究

Corresponding author: PAN Haobo, professor, Tel: 15986769058, E-mail: hb.pan@siat.ac.cn

作者简介: 梁涛，男，1992年生，博士

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https://www.ams.org.cn/CN/10.11900/0412.1961.2024.00181 或 https://www.ams.org.cn/CN/Y2026/V62/I4/669

表1 实时定量聚合酶链式反应(qRT-PCR)实验中相关基因的引物序列

图1 对纯Mg进行不同浓度H3BO3水热溶液处理后所得BA涂层表面形貌的SEM像

图2 对纯Mg进行不同浓度H3BO3水热溶液处理所得BA样品的电化学测试结果

图3 BA样品的抗菌性能

图4 未处理纯Mg、BA、BA + Alg、BA + Alg@Ca样品表面的水接触角和形貌及BA + Alg@Ca横截面的形貌和EDS面扫描图

图5 各样品的物相分析

图6 样品在磷酸盐缓冲(PBS)溶液中的浸泡实验结果

图7 未处理纯Mg、BA、BA + Alg和BA + Alg@Ca样品在PBS溶液中的电化学测试结果

图8 各样品在PBS溶液中的抗氧化性能测试

图9 各样品的细胞相容性测试结果

图10 未处理纯Mg、BA、BA + Alg和BA + Alg@Ca样品的体外抗菌性能

图11 未处理纯Mg、BA、BA + Alg和BA + Alg@Ca样品对巨噬细胞RAW264.7的免疫调控性能

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