INFLUENCE OF SURFACE MODIFICATION OF Ti BY FLUORINE ION-IMPLANTATION ON FORMATION AND EXPRESSION OF COLLAGEN-I ON OSTEOBLAST
LIU Huiying1;2;WANG Xuejin3;WANG Langping4;WANG Xiaofeng4;AI Hongjun1
1.Department of Prosthodontics; School of Stomatology; China Medical University
2.Department of Prosthodontics; School of Stomatology; Dalian Medical University
3.Department of Stomatology; Affiliated Zhongshan Hospital of Dalian University
4.State Key Laboratory of Advanced Welding Production Technology; Harbin Institute of Technology
Cite this article:
LIU Huiying WANG Xuejin WANG Langping WANG Xiaofeng AI Hongjun. INFLUENCE OF SURFACE MODIFICATION OF Ti BY FLUORINE ION-IMPLANTATION ON FORMATION AND EXPRESSION OF COLLAGEN-I ON OSTEOBLAST. Acta Metall Sin, 2008, 44(12): 1485-1490.
Commercial pure Ti discs were fluorine ion-implanted by plasma immersion ion implantation technique, and the morphology and chemical composition of the modified surface layers were characterized by SEM and XPS, respectively. MG-63 cells were seeded on the surfaces of modified Ti discs in 24-well cell culture plates at a density of 2x10 4 mL-1 and examined by the molecular biology technique for 6, 24 and 48 h. It was confirmed by immunofluorescent staining that the formation of collagen-I on the implanted Ti was significantly more than that on the pure Ti. Furthermore, the reverse transcription polymerase chain reaction and Western blotting showed that the expressions of mRNA and protein of collagen--I on the implanted Ti were fairly greater than those on the pure Ti. It can be concluded that the modified surface containing fluorine is beneficial to improve the biocompatibility of Ti.
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