<strong>Ga</strong>添加对<strong>304L</strong>不锈钢力学性能和抗菌性能的影响

doi:10.11900/0412.1961.2022.00351

金属学报

2024, Vol. 60

Issue (7): 890-900 DOI: 10.11900/0412.1961.2022.00351

研究论文

本期目录 | 过刊浏览

Ga添加对304L不锈钢力学性能和抗菌性能的影响

孟玉佳¹^,², 席通², 杨春光²(

), 赵金龙², 张新蕊², 于英杰², 杨柯²

1 中国科学技术大学材料科学与工程学院沈阳 110016
2 中国科学院金属研究所师昌绪先进材料创新中心沈阳 110016

Effect of Gallium Addition on Mechanical and Antibacterial Properties of 304L Stainless Steel

MENG Yujia¹^,², XI Tong², YANG Chunguang²(

), ZHAO Jinlong², ZHANG Xinrui², YU Yingjie², YANG Ke²

1 School of Materials Science and Engineering, University of Science and Technology of China, Shenyang 110016, China
2 Shi -changxu Advanced Materials Innovation Center, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China

引用本文:

孟玉佳, 席通, 杨春光, 赵金龙, 张新蕊, 于英杰, 杨柯. Ga添加对304L不锈钢力学性能和抗菌性能的影响[J]. 金属学报, 2024, 60(7): 890-900.
Yujia MENG, Tong XI, Chunguang YANG, Jinlong ZHAO, Xinrui ZHANG, Yingjie YU, Ke YANG. Effect of Gallium Addition on Mechanical and Antibacterial Properties of 304L Stainless Steel[J]. Acta Metall Sin, 2024, 60(7): 890-900.

全文: PDF(2800 KB) HTML

摘要:

Ga元素具有优异的抗菌能力，多形成镓配合物作为药物使用，但Ga很少在镓合金中展现其抗菌效果。为挖掘含Ga合金的抗菌能力，本工作利用金相观察、拉伸实验以及抗菌实验等方法探究Ga添加对304L奥氏体不锈钢的力学性能和抗菌性能的影响，并初步探讨了304L-Ga不锈钢的抗菌机理。结果表明，经过固溶处理后，Ga添加使不锈钢的屈服强度和伸长率提高，抗拉强度和硬度降低，同时因其固溶作用使不锈钢晶格常数增大。304L-Ga不锈钢具有优异的抗菌性，钝化膜表面释放的Ga³⁺引起细菌中活性氧(ROS)的高表达，导致氧化应激和杀菌作用。接触杀菌为304L-Ga不锈钢的杀菌机制之一，细菌与不锈钢充分接触，使细菌体内的质子(H⁺)与不锈钢表面所释放电子发生耗尽反应，促进不锈钢表面Ga的溶解。同时，在质子消耗反应中产生额外的ROS，进一步增强了材料的抗菌效果。

关键词 ：含Ga不锈钢, 微观组织, 力学性能, 接触抗菌

Abstract：

As a new antibacterial metal element, Ga is widely used in the medical field and always added to compounds in ionic form to form Ga complexes for medicinal use. However, related research on the mechanical properties, antibacterial properties, and antibacterial mechanism of Ga-bearing alloys is still very limited. In this work, the effect of Ga addition on the mechanical properties of 304L austenitic stainless steel (304L SS) after solution treatment was investigated via metallographic observations and tensile strength and hardness tests. Moreover, the antibacterial properties of Ga-bearing 304L stainless steel (304L-Ga SS) were tested using plate counting and the activity state of bacteria on the surface of the material was detected using SEM. Based on the known Ga ion sterilization principle, the antibacterial mechanism of 304L-Ga SS was preliminarily discussed using the reactive oxygen species (ROS) fluorescence reaction and ion dissolution results of the material in different solution tests. Results showed that the structure of 304L-Ga SS is still austenitic like that of 304L SS. The Ga addition increases the yield strength and elongation of the material but decreases its tensile strength and hardness. The change in strength and elongation is the result of the synergistic effect of the increase in stacking fault energy and the solid solution strengthening. The Ga addition also slightly increases the lattice constant of stainless steel due to the replacement solid solution effect. In the passive film of 304L-Ga SS, Ga exists in alloy form. Because of their similarity to Fe ions, Ga ions dissolved from Ga in the passive film are inhaled into bacteria cells and cause high expression of ROS in the bacteria, causing oxidative stress, and bactericidal effect. Contact sterilization is one of the main bactericidal mechanisms of 304L-Ga SS. Adequate contact between the bacteria and stainless steel improves the dissolution of Ga due to the proton (H⁺) depletion reaction in the bacteria. At the same time, the production of additional ROS during the proton consumption reaction further enhances the antibacterial effect.

Key words： Ga-bearing stainless steel microstructure mechanical property contact antibacterial

收稿日期: 2022-07-20

ZTFLH:

TG142.71

基金资助:中国国家自然科学基金会(52171242);佛山市中医院“登峰计划”项目(202000206);中国科学院青年创新促进会(2018221)

通讯作者: 杨春光，cgyang@imr.ac.cn，主要从事抗菌金属材料与应用研究

Corresponding author: YANG Chunguang, professor, Tel: (024)23971899, E-mail: cgyang@imr.ac.cn

作者简介: 孟玉佳，女，1996年生，硕士生

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表1 304L及304L-Ga不锈钢试样的化学成分 (mass fraction / %)

图1 拉伸试样的尺寸示意图

图2 304L和304L-Ga不锈钢显微组织的SEM像以及Ga在304L-Ga不锈钢中的EDS分布

图3 304L和304L-Ga不锈钢的XRD谱和高角度峰衍射谱

图4 304L和304L-Ga不锈钢的工程应变-应力曲线和力学性能

表2 304L和304L-Ga不锈钢力学相关参数

图5 E. coli和S. mutans与304L和304L-Ga不锈钢共培养后生长的菌落情况及304L-Ga不锈钢的抗菌率

图6 E. coli和S. mutans在304L和304L-Ga不锈钢的菌落形态

图7 E. coli和S. mutans在304L和304L-Ga不锈钢共培养24 h后的2',7'-二氯荧光素(DCF)荧光强度和丙二酫(MDA)生成含量

图8 304L-Ga不锈钢在37℃与不同溶液共培养24 h后析出的Ga3+浓度

图9 304L-Ga不锈钢在模拟唾液中浸泡前后表面Ga的化学状态

图10 304L-Ga不锈钢的抗菌机理示意图

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