PATTERNS OF CELL DEATH INDUCED BY FERRITE STAINLESS STEEL OF MAGNETIC ATTACHMENT IN THE MOUSE FIBROBLAST L929 CELLS

Acta Metall Sin

2006, Vol. 42

Issue (8): 892-896 DOI:

Research Articles

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PATTERNS OF CELL DEATH INDUCED BY FERRITE STAINLESS STEEL OF MAGNETIC ATTACHMENT IN THE MOUSE FIBROBLAST L929 CELLS

中国医科大学口腔医学院

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. PATTERNS OF CELL DEATH INDUCED BY FERRITE STAINLESS STEEL OF MAGNETIC ATTACHMENT IN THE MOUSE FIBROBLAST L929 CELLS. Acta Metall Sin, 2006, 42(8): 892-896 .

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Abstract The magnetic attachment wrapped with 26-1 ultra-purified ferrite stainless steel can improve its corrosion resistance. The fibroblast L929 cells of mouse were treated in vitro through 3 kinds of leaching liquors of stainless steel to detect the apoptosis rate and cell cycle distribution of L929 by the flow cytometry PI staining method, and the morphology of cell apoptosis was observed by electron microscope. Compared with the Tilite alloy and Co-Cr alloy, the type of cell apoptosis was quantitatively determined by di-parameter technique in which the annexin was marked by the fluorescein isothiocyanate (FIT) and stained by the iodonadine. The result showed that 26-1 ferrite stainless steel can induce the apoptosis of L929 cells with a fashion of time-dependence, and the apoptosis rate depends on the concentration of the leaching liquor. It also proved that 26-1 ferrite stainless steel meets the biocompatibility as a clinical material and can be safely applied in clinic.

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Received: 24 February 2006

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