铜绿假单胞菌对CrCoNi中熵合金微生物腐蚀行为的影响
收稿日期: 2019-01-29
修回日期: 2019-04-04
网络出版日期: 2019-04-30
基金资助
国家自然科学基金项目Nos(51434004);国家自然科学基金项目Nos(51774074);国家自然科学基金项目Nos(U1435205);国家自然科学基金项目Nos(51434004、51774074和U1435205);中央高校基本科研业务费专项基金项目No(N172512033);以及沈阳市重大科技成果转化项目No(Z17-5-003)
Investigation on Microbiologically Influenced Corrosion Behavior of CrCoNi Medium-Entropy Alloy byPseudomonas Aeruginosa
Received date: 2019-01-29
Revised date: 2019-04-04
Online published: 2019-04-30
Supported by
National Natural Science Foundation of China(51434004);National Natural Science Foundation of China(51774074);National Natural Science Foundation of China(U1435205);National Natural Science Foundation of China(51434004、51774074和U1435205);Fundamental Research Funds for the Central Universities No(N172512033);and Transformation Project of Major Scientific and Technological Achievements in Shenyang City(Z17-5-003)
采用多种电化学实验手段及场发射扫描电子显微镜(FESEM)、激光共聚焦扫描显微镜(CLSM)等分析技术，结合活死细菌染色实验、点蚀坑深度分析等方法，以316L不锈钢为对比，研究了CrCoNi中熵合金在含铜绿假单胞菌培养基中的微生物腐蚀行为。结果表明：铜绿假单胞菌能够在CrCoNi中熵合金表面形成不均匀的生物被膜，从而降低开路电位，减小极化电阻和电荷转移电阻，增大腐蚀电流密度；铜绿假单胞菌生物被膜在一定程度上破坏了钝化膜，导致浸泡在含铜绿假单胞菌培养基中的CrCoNi中熵合金的最大点蚀坑深度(4.8 μm)大于无菌培养基中CrCoNi中熵合金的最大点蚀坑深度(2.3 μm)。与316L不锈钢相比，CrCoNi中熵合金的开路电位较高，腐蚀电流密度和腐蚀速率较小，钝化膜的修复能力较强，在含铜绿假单胞菌培养基中浸泡后的最大点蚀坑深度小于316L不锈钢(5.8 μm)。
关键词： CrCoNi中熵合金; 铜绿假单胞菌; 微生物腐蚀; 生物被膜; 点蚀
冯浩 , 李花兵 , 路鹏冲 , 杨纯田 , 姜周华 , 武晓雷 . 铜绿假单胞菌对CrCoNi中熵合金微生物腐蚀行为的影响[J]. 金属学报, 2019 , 55(11) : 1457 -1468 . DOI: 10.11900/0412.1961.2019.00030
The CrCoNi medium-entropy alloy (MEA) has excellent strength and toughness, and can be used as the basis for the development of promising engineering alloys in the future. However, microbiologically influenced corrosion (MIC) of CrCoNi MEA has rarely been reported. Especially, pseudomonas aeruginosa (P. aeruginosa) is the typical bacteria associated with MIC, which is widely distributed in the ocean and soil. It can form biofilm on the surface of steel and accelerate the corrosion of carbon steels and stainless steels (SSs). In this study, the electrochemical experiments such as open current potential (OCP), linear polarization resistance (LPR), electrochemical frequency modulation (EFM), electrochemical impedance spectroscopy (EIS) and cyclic polarization (CP) were used to investigate the MIC behavior of CrCoNi MEA caused by P. aeruginosa, in comparison with 316L SS. Surface analysis techniques such as FESEM and CLSM were used to observe the P. aeruginosa biofilm and pitting morphology on the coupon surface. The results show that P. aeruginosa could form an uneven biofilm on the surface of CrCoNi MEA coupons. The P. aeruginosa accelerated the corrosion rate of CrCoNi MEA, which was demonstrated by a negative shift of open circuit potential, a decrease of polarization resistance and charge transfer resistance, and an increase of corrosion current density in P. aeruginosa medium. The P. aeruginosa biofilm could destroy the passive film of the CrCoNi MEA coupons, which led to the maximum pit depth of the coupons exposed in P. aeruginosa medium (4.8 μm) for 14 d much deeper than that in sterile medium (2.3 μm). Compared with 316L SS, CrCoNi had higher open circuit potential, lower corrosion current density and corrosion rate, and higher repairability of passive film. Meanwhile, the maximum pit depth on the CrCoNi MEA coupons in P. aeruginosa medium was shallower than that of 316L SS (5.8 μm).
| [1] | ZhangY, ZuoT T, TangZ, et al. Microstructures and properties of high-entropy alloys [J]. Prog. Mater. Sci., 2014, 61: 1 |
| [2] | GaoM C, QiaoJ W. High-entropy alloys (HEAs) [J]. Metals, 2018, 8: 108 |
| [3] | JiangH, JiangL, QiaoD X, et al. Effect of niobium on microstructure and properties of the CoCrFeNbxNi high entropy alloys [J]. J. Mater. Sci. Technol., 2017, 33: 712 |
| [4] | ZhangC, ZhangF, DiaoH Y, et al. Understanding phase stability of Al-Co-Cr-Fe-Ni high entropy alloys [J]. Mater. Des., 2016, 109: 425 |
| [5] | FengH, LiH B, WuX L, et al. Effect of nitrogen on corrosion behaviour of a novel high nitrogen medium-entropy alloy CrCoNiN manufactured by pressurized metallurgy [J]. J. Mater. Sci. Technol., 2018, 34: 1781 |
| [6] | MiaoJ W, GuoT M, RenJ F, et al. Optimization of mechanical and tribological properties of FCC CrCoNi multi-principal element alloy with Mo addition [J]. Vacuum, 2018, 149: 324 |
| [7] | GludovatzB, HohenwarterA, ThurstonK V S, et al. Exceptional damage-tolerance of a medium-entropy alloy CrCoNi at cryogenic temperatures [J]. Nat. Commun., 2016, 7: 10602 |
| [8] | Dan SathiarajG, SkrotzkiW, PukenasA, et al. Effect of annealing on the microstructure and texture of cold rolled CrCoNi medium-entropy alloy [J]. Intermetallics, 2018, 101: 87 |
| [9] | SloneC E, ChakrabortyS, MiaoJ, et al. Influence of deformation induced nanoscale twinning and FCC-HCP transformation on hardening and texture development in medium-entropy CrCoNi alloy [J]. Acta Mater., 2018, 158: 38 |
| [10] | MaY, YuanF P, YangM X, et al. Dynamic shear deformation of a CrCoNi medium-entropy alloy with heterogeneous grain structures [J]. Acta Mater., 2018, 148: 407 |
| [11] | LiuX W, LaplancheG, KostkaA, et al. Columnar to equiaxed transition and grain refinement of cast CrCoNi medium-entropy alloy by microalloying with titanium and carbon [J]. J. Alloys Compd., 2019, 775: 1068 |
| [12] | XuD K, LiY C, SongF M, et al. Laboratory investigation of microbiologically influenced corrosion of C1018 carbon steel by nitrate reducing bacterium Bacillus licheniformis [J]. Corros. Sci., 2013, 77: 385 |
| [13] | ZhangP Y, XuD K, LiY C, et al. Electron mediators accelerate the microbiologically influenced corrosion of 304 stainless steel by the Desulfovibrio vulgaris biofilm [J]. Bioelectrochemistry, 2015, 101: 14 |
| [14] | GuC X, XiaR, ZhuG J, et al. Study on corrosion of marine microbial of stainless steel [J]. Ship Eng., 2017, 39(10): 57 |
| [14] | 顾彩香, 夏 瑞, 朱冠军等. 不锈钢海洋微生物腐蚀研究 [J]. 船舶工程, 2017, 39(10): 57 |
| [15] | Wikie?A J, DatsenkoI, VeraM, et al. Impact of Desulfovibrio alaskensis biofilms on corrosion behaviour of carbon steel in marine environment [J]. Bioelectrochemistry, 2014, 97: 52 |
| [16] | XuD K, LiY C, GuT Y. Mechanistic modeling of biocorrosion caused by biofilms of sulfate reducing bacteria and acid producing bacteria [J]. Bioelectrochemistry, 2016, 110: 52 |
| [17] | ShiX B, XuD K, YanM C, et al. Study on microbiologically influenced corrosion behavior of novel Cu-bearing pipeline steels [J]. Acta Metall. Sin., 2017, 53: 153 |
| [17] | 史显波, 徐大可, 闫茂成等. 新型含Cu管线钢的微生物腐蚀行为研究 [J]. 金属学报, 2017, 53: 153 |
| [18] | WangM F, LiuH F, XuL M. Applied research on the competitive growth of bacteria in biological control of MIC [J]. J. Chin. Soc. Corros. Prot., 2004, 24: 159 |
| [18] | 汪梅芳, 刘宏芳, 许立铭. 细菌竞争生长在微生物腐蚀防治中的应用研究 [J]. 中国腐蚀与防护学报, 2004, 24: 159 |
| [19] | DongZ H, GuoX P, LiuH F, et al. Study on electrochemistry characteristics in MIC by wire beam electrodes [J]. J. Chin. Soc. Corros. Prot., 2002, 22: 48 |
| [19] | 董泽华, 郭兴蓬, 刘宏芳等. 用丝束电极研究SRB微生物诱导腐蚀的电化学特征 [J]. 中国腐蚀与防护学报, 2002, 22: 48 |
| [20] | XiaJ, YangC G, XuD K, et al. Laboratory investigation of the microbiologically influenced corrosion (MIC) resistance of a novel Cu-bearing 2205 duplex stainless steel in the presence of an aerobic marine Pseudomonas aeruginosa biofilm [J]. Biofouling, 2015, 31: 481 |
| [21] | LiH B, ZhouE Z, RenY B, et al. Investigation of microbiologically influenced corrosion of high nitrogen nickel-free stainless steel by Pseudomonas aeruginosa [J]. Corros. Sci., 2016, 111: 811 |
| [22] | LiH B, YangC T, ZhouE Z, et al. Microbiologically influenced corrosion behavior of S32654 super austenitic stainless steel in the presence of marine Pseudomonas aeruginosa biofilm [J]. J. Mater. Sci. Technol., 2017, 33: 1596 |
| [23] | ZhouE Z, LiH B, YangC T, et al. Accelerated corrosion of 2304 duplex stainless steel by marine Pseudomonas aeruginosa biofilm [J]. Int. Biodeterior. Biodegrad., 2018, 127: 1 |
| [24] | ZhaoY, ZhouE Z, XuD K, et al. Laboratory investigation of microbiologically influenced corrosion of 2205 duplex stainless steel by marine Pseudomonas aeruginosa biofilm using electrochemical noise [J]. Corros. Sci., 2018, 143: 281 |
| [25] | ShibataT. 1996 W.R.Whitney award lecture: Statistical and stochastic approaches to localized corrosion [J]. Corrosion, 1996, 52: 813 |
| [26] | MengG Z, WeiL Y, ZhangT, et al. Effect of microcrystallization on pitting corrosion of pure aluminium [J]. Corros. Sci., 2009, 51: 2151 |
| [27] | GholamiM, HoseinpoorM, MoayedM H. A statistical study on the effect of annealing temperature on pitting corrosion resistance of 2205 duplex stainless steel [J]. Corros. Sci., 2015, 94: 156 |
| [28] | ZhangT, ChenC M, ShaoY W, et al. Corrosion of pure magnesium under thin electrolyte layers [J]. Electrochim. Acta, 2008, 53: 7921 |
| [29] | ZhangT, LiuX L, ShaoY W, et al. Electrochemical noise analysis on the pit corrosion susceptibility of Mg-10Gd-2Y-0.5Zr, AZ91D alloy and pure magnesium using stochastic model [J]. Corros. Sci., 2008, 50: 3500 |
| [30] | MoradiM, SongZ L, YangL J, et al. Effect of marine Pseudoalteromonas sp. on the microstructure and corrosion behaviour of 2205 duplex stainless steel [J]. Corros. Sci., 2014, 84: 103 |
| [31] | VasylievG S. The influence of flow rate on corrosion of mild steel in hot tap water [J]. Corros. Sci., 2015, 98: 33 |
| [32] | AljohaniT A, HaydenB E. A simultaneous screening of the corrosion resistance of Ni-W thin film alloys [J]. Electrochim. Acta, 2013, 111: 930 |
| [33] | ZouY, WangJ, ZhengY Y. Electrochemical techniques for determining corrosion rate of rusted steel in seawater [J]. Corros. Sci., 2011, 53: 208 |
| [34] | MuX, WeiJ, DongJ H, et al. In situ corrosion monitoring of mild steel in a simulated tidal zone without marine fouling attachment by electrochemical impedance spectroscopy [J]. J. Mater. Sci. Technol., 2014, 30: 1043 |
| [35] | YuL B, YanM C, MaJ, et al. Sulfate reducing bacteria corrosion of pipeline steel in Fe-rich red soil [J]. Acta Metall. Sin., 2017, 53: 1568 |
| [35] | 于利宝, 闫茂成, 马 健等. 富Fe红壤中管线钢的硫酸盐还原菌腐蚀行为 [J]. 金属学报, 2017, 53: 1568 |
| [36] | YuanS J, ChoongA M F, PehkonenS O. The influence of the marine aerobic Pseudomonas strain on the corrosion of 70/30 Cu-Ni alloy [J]. Corros. Sci., 2007, 49: 4352 |
| [37] | LiY C, XuD K, ChenC F, et al. Anaerobic microbiologically influenced corrosion mechanisms interpreted using bioenergetics and bioelectrochemistry: A review [J]. J. Mater. Sci. Technol., 2018, 34: 1713 |
| [38] | HuangY, ZhouE Z, JiangC Y, et al. Endogenous phenazine-1-carboxamide encoding gene PhzH regulated the extracellular electron transfer in biocorrosion of stainless steel by marine Pseudomonas aeruginosa [J]. Electrochem. Commun., 2018, 94: 9 |
| [39] | VenzlaffH, EnningD, SrinivasanJ, et al. Accelerated cathodic reaction in microbial corrosion of iron due to direct electron uptake by sulfate-reducing bacteria [J]. Corros. Sci., 2013, 66: 88 |
| [40] | XuD K, GuT Y. Carbon source starvation triggered more aggressive corrosion against carbon steel by the Desulfovibriovulgaris biofilm [J]. Int. Biodeterior. Biodegrad., 2014, 91: 74 |
| [41] | ParkJ J, PyunS I. Stochastic approach to the pit growth kinetics of Inconel alloy 600 in Cl- ion-containing thiosulphate solution at temperatures 25—150 ℃ by analysis of the potentiostatic current transients [J]. Corros. Sci., 2004, 46: 285 |
/
| 〈 |
|
〉 |
