Ga添加对304L不锈钢力学性能和抗菌性能的影响
收稿日期: 2022-07-20
修回日期: 2022-09-01
网络出版日期: 2022-09-15
基金资助
中国国家自然科学基金会(52171242);佛山市中医院“登峰计划”项目(202000206);中国科学院青年创新促进会(2018221)
Effect of Gallium Addition on Mechanical and Antibacterial Properties of 304L Stainless Steel
Received date: 2022-07-20
Revised date: 2022-09-01
Online published: 2022-09-15
Supported by
National Natural Science Foundation of China(52171242);Peak Climbing Project of Foshan Hospital of Traditional Chinese Medicine(202000206);Youth Innovation Promotion Association CAS(2018221)
Ga元素具有优异的抗菌能力，多形成镓配合物作为药物使用，但Ga很少在镓合金中展现其抗菌效果。为挖掘含Ga合金的抗菌能力，本工作利用金相观察、拉伸实验以及抗菌实验等方法探究Ga添加对304L奥氏体不锈钢的力学性能和抗菌性能的影响，并初步探讨了304L-Ga不锈钢的抗菌机理。结果表明，经过固溶处理后，Ga添加使不锈钢的屈服强度和伸长率提高，抗拉强度和硬度降低，同时因其固溶作用使不锈钢晶格常数增大。304L-Ga不锈钢具有优异的抗菌性，钝化膜表面释放的Ga3+引起细菌中活性氧(ROS)的高表达，导致氧化应激和杀菌作用。接触杀菌为304L-Ga不锈钢的杀菌机制之一，细菌与不锈钢充分接触，使细菌体内的质子(H+)与不锈钢表面所释放电子发生耗尽反应，促进不锈钢表面Ga的溶解。同时，在质子消耗反应中产生额外的ROS，进一步增强了材料的抗菌效果。
孟玉佳 , 席通 , 杨春光 , 赵金龙 , 张新蕊 , 于英杰 , 杨柯 . Ga添加对304L不锈钢力学性能和抗菌性能的影响[J]. 金属学报, 2024 , 60(7) : 890 -900 . DOI: 10.11900/0412.1961.2022.00351
As a new antibacterial metal element, Ga is widely used in the medical field and always added to compounds in ionic form to form Ga complexes for medicinal use. However, related research on the mechanical properties, antibacterial properties, and antibacterial mechanism of Ga-bearing alloys is still very limited. In this work, the effect of Ga addition on the mechanical properties of 304L austenitic stainless steel (304L SS) after solution treatment was investigated via metallographic observations and tensile strength and hardness tests. Moreover, the antibacterial properties of Ga-bearing 304L stainless steel (304L-Ga SS) were tested using plate counting and the activity state of bacteria on the surface of the material was detected using SEM. Based on the known Ga ion sterilization principle, the antibacterial mechanism of 304L-Ga SS was preliminarily discussed using the reactive oxygen species (ROS) fluorescence reaction and ion dissolution results of the material in different solution tests. Results showed that the structure of 304L-Ga SS is still austenitic like that of 304L SS. The Ga addition increases the yield strength and elongation of the material but decreases its tensile strength and hardness. The change in strength and elongation is the result of the synergistic effect of the increase in stacking fault energy and the solid solution strengthening. The Ga addition also slightly increases the lattice constant of stainless steel due to the replacement solid solution effect. In the passive film of 304L-Ga SS, Ga exists in alloy form. Because of their similarity to Fe ions, Ga ions dissolved from Ga in the passive film are inhaled into bacteria cells and cause high expression of ROS in the bacteria, causing oxidative stress, and bactericidal effect. Contact sterilization is one of the main bactericidal mechanisms of 304L-Ga SS. Adequate contact between the bacteria and stainless steel improves the dissolution of Ga due to the proton (H+) depletion reaction in the bacteria. At the same time, the production of additional ROS during the proton consumption reaction further enhances the antibacterial effect.
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