Effect of β-fleck on properties of tensile elongation and low cycle fatigue life at room temperature for Ti-10V-2Fe-3Al alloy has been investigated. The cracks along initial β-grain boundaries in the β-fleck region may propagate to form intergranular brittle fracture. Under alternating load, the β-fleck often becomes a fatigue origin. While under higher strain, the cracks initiate and propagate to fracture early at the original β-grain boundaries in β-fleck region and at α-grain boundaries.