The Microstructure and in Vivo and in Vitro Property of Multi-Component Composite Films on the Biomedical Pure Magnesium Surface

doi:10.11900/0412.1961.2017.00243

Acta Metall Sin

2017, Vol. 53

Issue (10): 1337-1346 DOI: 10.11900/0412.1961.2017.00243

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The Microstructure and in Vivo and in Vitro Property of Multi-Component Composite Films on the Biomedical Pure Magnesium Surface

Muqin LI(

), Haitao YAO, Fanghong WEI, Mingda LIU, Zan WANG, Shuhao PENG

Key Laboratory of Biomaterials of Heilongjiang Province, Jiamusi University, Jiamusi 154007, China

Cite this article:

Muqin LI, Haitao YAO, Fanghong WEI, Mingda LIU, Zan WANG, Shuhao PENG. The Microstructure and in Vivo and in Vitro Property of Multi-Component Composite Films on the Biomedical Pure Magnesium Surface. Acta Metall Sin, 2017, 53(10): 1337-1346.

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Abstract

Entering into 21 Century,the degradable Mg and Mg alloy become research focus for the development of Internal fixation material from inert to active metal. The polybasic coating (UMAO-OH-SCA-SF) was prepared by ultrasound micro-arc oxidation (UMAO), alkali treatment (OH), treatment of silicohydride conversion coating (SCA) and the treatment of self-assembly silk fibroin on the surface of magnesium, which can improve the corrosion resistance and bioactivity of pure magnesium. The surface topography, structure, corrosion resistance, cell activity and bone growth in vivo of the coating were studied by SEM, IR spectra, electrochemical measurement, vitro experiment and implant test. The results show that the main phase of coating is MgO. The alkali treatment is beneficial to forming Si-O-Mg film by silicohydride coupling. With the self-assembly silk fibroin time increasing, the silk fibroin structure changes from random coil to β-fold. The polybasic coating self-corrosion is improved and self-corrosion current is reduced by two orders of magnitude. Compared to substrate, the polybasic coating has better proliferation, adherent and differentiation of osteoblast. It has a better bone integration capacity in the bone healing early stage, and which can control magnesium ion dissolving. The UMAO-OH-SCA-SF/1.5h coating has the best property.

Key words: pure magnesium ultrasound micro-arc oxidation silicohydride silk fibroin corrosion resistance bioactivity

Received: 19 June 2017

ZTFLH:

R318.08

Fund: Supported by National Natural Science Foundation of China (No.31370979)

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	Muqin LI
	Haitao YAO
	Fanghong WEI
	Mingda LIU
	Zan WANG
	Shuhao PENG

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https://www.ams.org.cn/EN/10.11900/0412.1961.2017.00243 OR https://www.ams.org.cn/EN/Y2017/V53/I10/1337

Fig.1 Pure Mg screw implantation (a) and sampling on the mandible (b)

Fig.2 Surface and section SEM images of different coatings
(a) UMAO (b) UMAO-OH-SCA-SF/0.5h
(c) UMAO-OH-SCA-SF/1.0h (d) UMAO-OH-SCA-SF/1.5h

Fig.3 Element contents of the different coatings
(a) UMAO (b) UMAO-OH-SCA-SF/0.5h
(c) UMAO-OH-SCA-SF/1.0h (d) UMAO-OH-SCA-SF/1.5h

Fig.4 Infrared spectra of coatings with different treatment time

Fig.5 Contact angles of coatings with different treatment times
(a) UMAO (b) UMAO-OH-SCA-SF/0.5h
(c) UMAO-OH-SCA-SF/1.0h
(d) UMAO-OH-SCA-SF/1.5h

Fig.6 Tafel polarization curves of coatings with different treatment times

Coating	E_corr / V	i_corr 10^-5Acm^-2
UMAO	-1.554 $±$ 0.10	211.3 $±$ 8.2
UMAO-OH-SCA-SF/0.5h	-1.482 $±$ 0.11	6.577 $±$ 1.2
UMAO-OH-SCA-SF/1.0h	-1.461 $±$ 0.15	3.595 $±$ 0.91
UMAO-OH-SCA-SF/1.5h	-1.447 $±$ 0.10	2.135 $±$ 0.51

Table 1 Corrosion potential (E_corr) and corrosion current density (i_corr) of coatings with different treatment times in NaCl solution

Fig.7 Osteoblast proliferation by CCK8 testing (a) and activity ALP testing (b) of different coatings

Fig.8 Hematoxylin eosin (HE) staining of different coatings around the implantation of UMAO (a1~a3), UMAO-OH-SCA-SF/0.5h (b1~b3) and UMAO-OH-SCA-SF/1.5h (c1~c3) for 2 weeks (a1~c1), 4 weeks (a2~c2) and 6 weeks (a3~c3)

Fig.9 BMP-2 average gray level testing on the coatings of different implantation-bone osseointegration interface

Fig.10 variation concentrations of Mg²⁺ in the animal serum with implant of different coatings

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